Silver-stained fibrin zymography: separation of proteases and activity detection using a single substrate-containing gel

Mixed-substrate (glycerol tributyrate an

Mixed-substrate (glycerol tributyrate and fibrin) zymography for simultaneous detection of lipolytic and proteolytic enzymes on a single gel

✍ Nack-Shick Choi; Jong Hyun Choi; Bo-Hye Kim; Yun-Jon Han; Joong Su Kim; Seung-Go 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 184 KB

## Abstract A new zymography method for simultaneous detection of two different enzymatic activities (lipolytic and proteolytic) using a single SDS‐containing or native‐conformation gel and a mixed‐substrate (glycerol tributyrate and fibrin) (MS)^1^ gel was developed. After routine electrophoresis,