Sensitive fluorogenic substrates for the detection of trypsinlike proteases and pancreatic elastase

✍ Scribed by Paul J. Brynes; Patricia Andrade; David Gordon

• Book ID: 102985878
• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 734 KB
• Volume: 126
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90542-5

✦ Synopsis

Several new fluorogenic substrates capable of measuring the proteolytic activities of pancreatic elastase, trypsin, and trypsinlike enzymes were prepared. The fluorophore utilized in these substrates is 6-aminoquinoline (6-AQ), a leaving group that affords significant improvement over the use of other chromogenic moieties in that (i) the appearance of 6-AQ can be measured fluorimetrically at its excitation and emission maxima, while at these wavelengths the substrate is essentially nonfluorescent; (ii) the reduction of background emission confers an enhanced detection limit to the substrate; and (iii) the amino group of 6-AQ very readily undergoes acylation by peptides and enables substrates to be synthesized in good yields.