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Retrovirus-mediated gene expression in mammalian cells

โœ Scribed by William R.A. Osborne

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
476 KB
Volume
2
Category
Article
ISSN
0958-1669

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โœฆ Synopsis

Significant advances have been made in precisely defining the elements in the Moloney murine leukemia virus genome responsible for tissue-restricted expression. This knowledge should lead to improved expression vectors for gene transfer in mammalian cells. In the past year, retrovirus-mediated gene expression in a diverse range of cell types has been reported. These cells have been used to study gene transfer relevant to a range of inherited diseases.

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## Abstract A quantitative understanding of the process of retrovirusโ€mediated gene transfer into mammalian cells should assist the design and optimization of transduction protocols. We present a mathematical model of the process that incorporates the essential rateโ€limiting transduction steps incl