Retinal Degeneration: Methods and Protocols

✍ Scribed by Bernhard H F Weber; Thomas Langmann

Publisher: Humana Press
Year: 2018
Tongue: English
Leaves: 413
Edition: Hardcover
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This volume provides key updates on several methods from the first edition as well as including new novel techniques to address the most recent technological developments and their applications in retinal research Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment approaches. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge,Retinal Degeneration: Methods and Protocols, Second Editionaims to ensure successful scientific work in the further study of this vital field.

✦ Table of Contents

Front Matter ....Pages i-xiv
Front Matter ....Pages 1-1
Identification and Analysis of Genes Associated with Inherited Retinal Diseases (Mubeen Khan, Zeinab Fadaie, Stéphanie S. Cornelis, Frans P. M. Cremers, Susanne Roosing)....Pages 3-27
Conduct and Quality Control of Differential Gene Expression Analysis Using High-Throughput Transcriptome Sequencing (RNASeq) (Felix Grassmann)....Pages 29-43
Testing for Known Retinal Degeneration Mutants in Mouse Strains (Khalid Rashid, Katharina Dannhausen, Thomas Langmann)....Pages 45-58
CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo (Daniela Benati, Valeria Marigo, Alessandra Recchia)....Pages 59-74
Monitoring Surface Reactions by Combined Western Blot-ELISA Analysis (Yuchen Lin, Sarah Irmscher, Christine Skerka)....Pages 75-83
Front Matter ....Pages 85-85
Generation of Functional Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cells (Caroline Brandl)....Pages 87-94
Advanced Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture (Francesca Mazzoni, Yingyu Mao, Silvia C. Finnemann)....Pages 95-108
Porcine RPE/Choroidal Explant Cultures (Alexa Klettner, Yoko Miura)....Pages 109-118
The Mouse Retinal Organoid Trisection Recipe: Efficient Generation of 3D Retinal Tissue from Mouse Embryonic Stem Cells (Manuela Völkner, Thomas Kurth, Mike O. Karl)....Pages 119-141
Cell Death Analysis in Retinal Cultures (Sarah L. Roche, Ana M. Ruiz-Lopez, Thomas G. Cotter)....Pages 143-152
Fate Mapping In Vivo to Distinguish Bona Fide Microglia Versus Recruited Monocyte-Derived Macrophages in Retinal Disease (Nancy J. Reyes, Rose Mathew, Daniel R. Saban)....Pages 153-164
Front Matter ....Pages 165-165
Light Damage Models of Retinal Degeneration (Christian Grimm, Charlotte E. Remé)....Pages 167-178
Induction and Readout of Oxygen-Induced Retinopathy (Raffael Liegl, Claudia Priglinger, Andreas Ohlmann)....Pages 179-191
Generation and Analysis of Xenopus laevis Models of Retinal Degeneration Using CRISPR/Cas9 (Joanna M. Feehan, Paloma Stanar, Beatrice M. Tam, Colette Chiu, Orson L. Moritz)....Pages 193-207
Gene Knockdown in Zebrafish (Danio rerio) as a Tool to Model Photoreceptor Diseases (Holger Dill, Utz Fischer)....Pages 209-219
Drosophila melanogaster: A Valuable Genetic Model Organism to Elucidate the Biology of Retinitis Pigmentosa (Malte Lehmann, Elisabeth Knust, Sarita Hebbar)....Pages 221-249
Front Matter ....Pages 251-251
Retinal Fundus Imaging in Mouse Models of Retinal Diseases (Anne F. Alex, Maged Alnawaiseh, Peter Heiduschka, Nicole Eter)....Pages 253-283
Phenotyping of Mouse Models with OCT (G. Alex Ochakovski, M. Dominik Fischer)....Pages 285-291
Cell-Specific Markers for the Identification of Retinal Cells and Subcellular Organelles by Immunofluorescence Microscopy (Laurie L. Molday, Christiana L. Cheng, Robert S. Molday)....Pages 293-310
Immuno-TEM/STEM in Retinal Research (Sanae Sakami, Krzysztof Palczewski)....Pages 311-332
Noninvasive Two-Photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium (Grazyna Palczewska, Timothy S. Kern, Krzysztof Palczewski)....Pages 333-343
Analysis of the Drosophila Compound Eye with Light and Electron Microscopy (Monalisa Mishra, Elisabeth Knust)....Pages 345-364
Front Matter ....Pages 365-365
Cell-Based Therapy for Retinal Disease: The New Frontier (Marco Zarbin)....Pages 367-381
In Vitro Evaluation of AAV Vectors for Retinal Gene Therapy (Johanna E. Wagner, Christian Schön, Elvir Becirovic, Martin Biel, Stylianos Michalakis)....Pages 383-390
Nanoparticles Targeting Retinal and Choroidal Capillaries In Vivo (Alexandra Haunberger, Achim Goepferich)....Pages 391-404
Optimized Subretinal Injection Technique for Gene Therapy Approaches (Regine Mühlfriedel, Stylianos Michalakis, Marina Garcia Garrido, Vithiyanjali Sothilingam, Christian Schön, Martin Biel et al.)....Pages 405-412
Back Matter ....Pages 413-417

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