Retinal Development: Methods and Protocols

✍ Scribed by Chai-An Mao

Publisher: Springer US;Humana
Year: 2020
Tongue: English
Leaves: 241
Series: Methods in Molecular Biology 2092
Edition: 1st ed. 2020
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This volume details commonly used molecular and cellular techniques and specialized methodologies for studying retina neuronal subtypes and electrophysiology. Chapters describe techniques for anatomical studies of retinal ganglion cell morphology, gap-junction-mediated neuronal connection, multi-electrode array recording on mouse retinas, and paired recording to study the electrical coupling between photoreceptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Retinal Development: Methods and Protocols aims to provide readers with a set of practical experimental tools to study retinal development, regeneration, and function of mature retinal neurons. Many of the protocols and strategies described in one organism can be easily adapted to applications in different model systems.

✦ Table of Contents

Front Matter ....Pages i-xi
Investigating Neurogenesis in Birds (Tania Rodrigues, Laurent Brodier, Jean-Marc Matter)....Pages 1-18
Studying In Vivo Retinal Progenitor Cell Proliferation in Xenopus laevis (Cindy X. Kha, Dylan J. Guerin, Kelly Ai-Sun Tseng)....Pages 19-33
Three-Dimensional Culture of Mouse Eyecups (Raven Diacou, Punita Bhansali, Wei Liu)....Pages 35-43
Live Imaging of Mouse Retinal Slices (Anthony P. Barrasso, Ross A. Poché)....Pages 45-53
Retinal Strip Culture for Studying Ganglion Cell Axon Growth (Masayuki Yamashita)....Pages 55-64
Multiple Approaches for Enhancing Neural Activity to Promote Neurite Outgrowth of Retinal Explants (Chuan-Chin Chiao, Chin-I Lin, Meng-Jung Lee)....Pages 65-75
Adeno-Associated Virus as Gene Delivery Vehicle into the Retina (Shuyun Deng, Kazuhiro Oka)....Pages 77-90
Transposon-Mediated Stable Suppression of Gene Expression in the Developing Chick Retina (Masaru Nakamoto, Chizu Nakamoto)....Pages 91-108
A Simple Guide for Generating BAC Transgenic Animals for Retinal Research (Cavit Agca, Christian Grimm)....Pages 109-122
Identification and Characterization of Cis-Regulatory Elements for Photoreceptor-Type-Specific Transcription in ZebraFish (Wei Fang, Yi Wen, Xiangyun Wei)....Pages 123-145
Ultrasensitive RNAscope In Situ Hybridization System on Embryonic and Adult Mouse Retinas (Takae Kiyama, Chai-An Mao)....Pages 147-158
Single-Cell Capture, RNA-seq, and Transcriptome Analysis from the Neural Retina (Rachayata Dharmat, Sangbae Kim, Yumei Li, Rui Chen)....Pages 159-186
Genetically Directed Sparse Labeling System for Anatomical Studies of Retinal Ganglion Cells (Leila Jamal, Takae Kiyama, Chai-An Mao)....Pages 187-194
Intracellular Dye Microinjection in Retinal Morphological and Communication Studies (Ye Long)....Pages 195-206
Multielectrode Array Recording of Mouse Retinas Transplanted with Stem Cell-Derived Retinal Sheets (Hung-Ya Tu, Take Matsuyama)....Pages 207-220
Paired Recording to Study Electrical Coupling Between Photoreceptors in Mouse Retina (Nange Jin, Zhijing Zhang, Kimberly A. Mankiewicz, Christophe P. Ribelayga)....Pages 221-230
Back Matter ....Pages 231-235

✦ Subjects

Life Sciences; Developmental Biology; Neurosciences

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