Axon Degeneration: Methods and Protocols

✍ Scribed by Elisabetta Babetto

• Publisher: Springer US;Humana
• Year: 2020
• Tongue: English
• Leaves: 340
• Series: Methods in Molecular Biology 2143
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This book is a collection of classical as well as innovative methods used to investigate axon degeneration with a particular focus on addressing the common challenges encountered while performing these procedures. Particular attention is devoted to the study of axon loss in several model organisms, as each poses unique challenges and provides powerful advantages. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Axon Degeneration: Methods and Protocols is an ideal guide for facilitating the application and further development of these protocols, which will help the scientific community tackle important questions regarding axon degeneration.
Chapters 2, 3, and 20 are available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.

✦ Table of Contents

Front Matter ....Pages i-xi
Front Matter ....Pages 1-1
Axon Degeneration: Which Method to Choose? (Michael P. Coleman)....Pages 3-12
Front Matter ....Pages 13-13
Axon Degeneration Assays in Superior Cervical Ganglion Explant Cultures (Andrea Loreto, Jonathan Gilley)....Pages 15-24
Microinjection of Superior Cervical Ganglion Neurons for Studying Axon Degeneration (Jonathan Gilley, Andrea Loreto)....Pages 25-39
Assessing Axonal Degeneration in Embryonic Dorsal Root Ganglion Neurons In Vitro (Jung Eun Shin, Yongcheol Cho)....Pages 41-54
Viral Transduction of DRG Neurons (Yo Sasaki)....Pages 55-62
Planning and Analysis of Axon Degeneration Screening Experiments (Lyndah Lovell, John Bramley, William Buchser)....Pages 63-82
A Microfluidic Culture Platform to Assess Axon Degeneration (Yu Yong, Christopher Hughes, Christopher Deppmann)....Pages 83-96
A Schwann Cell–Neuron Coculture System to Study Neuron–Glia Interaction During Axon Degeneration (Elisabetta Babetto)....Pages 97-110
Establishing Myelinating Cocultures Using Human iPSC-Derived Sensory Neurons to Investigate Axonal Degeneration and Demyelination (Alex J. Clark)....Pages 111-129
Front Matter ....Pages 131-131
Preparation of Organotypic Hippocampal Slice Cultures for the Study of CNS Disease and Damage (Claire S. Durrant)....Pages 133-144
Organotypic Culture Assay for Neuromuscular Synaptic Degeneration and Function (Kosala N. Dissanayake, Robert Chang-Chih Chou, Rosalind Brown, Richard R. Ribchester)....Pages 145-157
Intracellular Recordings of Postsynaptic Voltage Responses at the Drosophila Neuromuscular Junction (Vera Valakh, Johanna G. Flyer-Adams)....Pages 159-168
Ex Vivo Studies of Optic Nerve Axon Electrophysiology (Chinthasagar Bastian, Sylvain Brunet, Selva Baltan)....Pages 169-177
Ex Vivo Analysis of Axonal Degeneration Using Sciatic and Optic Nerve Preparations (Rodrigo López-Leal, Felipe A. Court)....Pages 179-189
Measuring Bioenergetic Signatures of Peripheral Nerve Segments by Extracellular Flux Analysis (Bogdan Beirowski)....Pages 191-203
Front Matter ....Pages 205-205
Detection of Neutrophils in the Sciatic Nerve Following Peripheral Nerve Injury (Jon P. Niemi, Jane A. Lindborg, Richard E. Zigmond)....Pages 207-222
Whole-Tissue Immunolabeling and 3D Fluorescence Imaging to Visualize Axon Degeneration in the Intact, Unsectioned Mouse Tissues (Ying Cao, Jing Yang)....Pages 223-232
Transmission Electron Microscopy and Morphometry of the CNS White Matter (Julia M. Edgar, Rebecca Sherrard Smith, Ian D. Duncan)....Pages 233-261
In Vivo Calcium Imaging During Axon Degeneration in Zebrafish (Mauricio E. Vargas)....Pages 263-270
In Vivo Imaging of Anterograde and Retrograde Axonal Transport in Rodent Peripheral Nerves (James N. Sleigh, Andrew P. Tosolini, Giampietro Schiavo)....Pages 271-292
In Vivo Visualization of Moving Synaptic Cargo Complexes within Drosophila Larval Segmental Axons (Rupkatha Banerjee, Joseph A. White II, Shermali Gunawardena)....Pages 293-300
A Chemotherapy-Induced Peripheral Neuropathy Model in Drosophila melanogaster (Martha R. C. Bhattacharya)....Pages 301-310
Models of Axon Degeneration in Drosophila Larvae (E. J. Brace, Aaron DiAntonio)....Pages 311-320
In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster (Mary A. Logan, Sean D. Speese)....Pages 321-338
Back Matter ....Pages 339-340

✦ Subjects

Life Sciences; Neurobiology; Neurosciences