Restriction map of the cya region of the Escherichia coli K12 chromosome
β Scribed by Roy, A.; Danchin, A.
- Book ID
- 123192761
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 349 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0300-9084
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The cya gene region of Escherichia coli K12 has been cloned into plasmid pBR322. Detailed analysis of the locus, using in vitro recombination techniques as well as specific labelling of gene products has given information on the organization and products of the region. The cya gene is preceded by tw
Small multicopy plasmids carrying the Escherichia coli genes ksgA and pdxA were constructed by ligation in vitro of an EcoRI restriction fragment from lambda ksg10 (AndrΓ©sson and Davies, 1980a) into the EcoRI sites of the ColE1 plasmids RSF2124 and pVH51. Cleavage maps of the plasmids were determine