๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

The cya locus of escherichia coli K12: Organization and gene products

โœ Scribed by Roy, A. ;Danchin, A.

Publisher
Springer
Year
1982
Tongue
English
Weight
867 KB
Volume
188
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

โœฆ Synopsis

The cya gene region of Escherichia coli K12 has been cloned into plasmid pBR322. Detailed analysis of the locus, using in vitro recombination techniques as well as specific labelling of gene products has given information on the organization and products of the region. The cya gene is preceded by two DNA segments which behave as promoters of equivalent strength; a divergent, very strong, promoter is also present in the same control region. The former pair of promoters direct transcription of the cya gene which is expressed as a 95,000 dalton polypeptide; the latter promoter controls synthesis of a 40,000 dalton polypeptide. The cya gene product can complement a cya delta mutation when fully expressed or when expressed as a protein truncated at its carboxy terminal end.

๐Ÿ“œ SIMILAR VOLUMES

The recN locus of Escherichia coli K12:
The recN locus of Escherichia coli K12: molecular analysis and identification of the gene product
โœ Picksley, Steven M. ;Morton, Stuart J. ;Lloyd, Robert G. ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Springer ๐ŸŒ English โš– 911 KB

The recN gene which is necessary for inducible DNA repair and recombination in Escherichia coli has been cloned into the low copy plasmid vector pHSG415. Analysis of the recombinant plasmid, pSP100, revealed a 5.6 Kb HindIII insert of chromosomal DNA. Transposon inactivation of recN function and ana

Organization and expression of the dnaJ
Organization and expression of the dnaJ and dnaK genes of Escherichia coli K12
โœ Saito, Haruo ;Uchida, Hisao ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Springer ๐ŸŒ English โš– 755 KB

A temperature-sensitive mutation in the dnaJ gene of Escherichia coli K12 is described which affects replication of the bacterial DNA. The gene is located adjacent to the dnaK gene described previously (Saito and Uchida, 1977). The physical and functional organization of the dnaJ-dnaK region was stu

Autoregulation of the rho gene of Escher
Autoregulation of the rho gene of Escherichia coli K-12
โœ Kung, Hsiang-fu ;Bekesi, Eva ;Guterman, Sonia K. ;Gray, John E. ;Traub, Lisa ;Ca ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Springer ๐ŸŒ English โš– 407 KB

It has previously been proposed, based on indirect evidence, that the Rho protein may control the expression of the rho gene. Using an in vitro system for the transcription and translation of the rho gene cloned into plasmid pBR322, we tested this hypothesis directly by monitoring the effect in vitr

Nucleotide sequence of the hemB gene of
Nucleotide sequence of the hemB gene of Escherichia coli K12
โœ Echelard, Yann ;Dymetryszyn, Julien ;Drolet, Marc ;Sasarman, A. ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Springer ๐ŸŒ English โš– 811 KB

The hemB gene of Escherichia coli K12, coding for porphobilinogen synthase (PBG-S; syn., 5-aminolevulinic acid dehydratase, ALA-D), was cloned following insertion of an EcoRI fragment of plasmid F'13 into the mobilizable vector pCR1. The hybrid plasmid carrying the hemB gene was able to complement a

Molecular cloning of the fnr gene of Esc
Molecular cloning of the fnr gene of Escherichia coli K12
โœ Shaw, Duncan J. ;Guest, John R. ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 594 KB

Mutations in the fnr gene of Escherichia coli have pleiotrophic effects leading to deficiencies in the reduction of fumarate and nitrate, hydrogen production and the ability to grow anaerobically with fumarate or nitrate as terminal electron acceptors. Transducing phages (lambda fnr) carrying the wi