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Genetic organization and restriction enzyme cleavage map of the ksgA-pdxA region of the Escherichia coli chromosome

✍ Scribed by Andresson, Ólafur S. ;Davies, Julian E.

Publisher
Springer
Year
1980
Tongue
English
Weight
932 KB
Volume
179
Category
Article
ISSN
0026-8925

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✦ Synopsis

Small multicopy plasmids carrying the Escherichia coli genes ksgA and pdxA were constructed by ligation in vitro of an EcoRI restriction fragment from lambda ksg10 (Andrésson and Davies, 1980a) into the EcoRI sites of the ColE1 plasmids RSF2124 and pVH51. Cleavage maps of the plasmids were determined for 21 different restriction enzymes. The ksgA gene was located in a 750 basepairs (bp) region 1,450 bp clockwise of the EcoRI site in folA: pdxA is in a 2,040 bp region immediately clockwise of ksgA.

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