Resting State in Normal and Simian Virus 40 Transformed Chinese Hamster Lung Cells
β Scribed by Robert G. Martin and Stefanie Stein
- Book ID
- 123654005
- Publisher
- National Academy of Sciences
- Year
- 1976
- Tongue
- English
- Weight
- 842 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/65970
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## Abstract Chinese hamster embryo cells transformed with the tsA 58 mutant of Simian virus 40 express the transformed phenotype at the permissive temperature (33Β°C or 37Β°C) and a βnormalβ phenotype at the nonpermissive temperature (40.5Β°C). Immunofluorescence and immunoprecipitation of T antigens
## Abstract Primary and secondary Chinese hamster (ChH) embryo cells infected with SV40 exhibited chromosomal changes within one cell generation (24 h). The initial change seen is an increase in the number of polyploid metaphases (>10%). The majority of the polyploid cells are tetraploid (8Γ), howe