𝔖 Bobbio Scriptorium
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Responses of fish chromatophore-based cytosensor to a broad range of biological agents

✍ Scribed by Karen P. Dierksen; Ljiljana Mojovic; Bruce A. Caldwell; R. Ryan Preston; Rosalyn Upson; Jeannine Lawrence; Philip N. McFadden; Janine E. Trempy

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
268 KB
Volume
24
Category
Article
ISSN
0260-437X

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✦ Synopsis

Abstract

A cytosensor based on living chromatophores from Betta splendens Siamese fighting fish was used to test several classes of biologically active agents. Tested agents include neurotransmitters, adenyl cyclase activators, cytoskeleton effectors, cell membrane effectors and protein synthesis inhibitors. Characteristic cell responses were analyzed, and potential cytosensor applications were considered. Streptococcus pyogenes toxins streptolysin S and streptolysin O, Clostridium tetani tetanolysin, Staphylococcus aureus alpha‐toxin and Vibrio parahemolyticus hemolysin, all bacterial toxins that act on cell membranes, elicited a strong response from chromatophores. A comparison of purified toxin to actual bacterial culture from Vibrio parahemolyticus demonstrated a nearly identical chromatophore cell response pattern. This suggests that the cytosensor response is reflective of bacterial toxin production. Copyright © 2004 John Wiley & Sons, Ltd.

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