✦ LIBER ✦

In vitro responses of human prostate tumour cell lines to a range of antitumour agents

✍ Scribed by Sylvia A. Metcalfe; Richard D. H. Whelan; John R. W. Masters; Bridget T. Hill

Publisher: John Wiley and Sons
Year: 1983
Tongue: French
Weight: 800 KB
Volume: 32
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910320315

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Four human prostate tumour continuous cell lines were employed for in vitro drug sensitivity testing of nine cytotoxic drugs, X‐irradiation and two hormonal preparations. Responses were assessed by measuring cell survival as judged by colony formation in agarose. A marked heterogeneity of responses was noted to many of the agents studied, including X‐irradiation. However, in terms of ID~70~ values for 24‐h drug exposures, comparable responses in all the cell lines tested, with values falling within a 1.4‐ to 2.9‐fold range, were noted with actinomycin D, adriamycin, m‐AMSA^4^, dibromodulcitol, 5‐fluorouracil, hydroxyurea and ICRF‐159. In contrast, mixed responses to cis‐platinum and methotrexate were particularly marked, with ID~70~ values differing by factors of 3.8 and 7.5 respectively. In addition, when higher drug concentrations of m‐AMSA, cis‐platinum, dibromodulcitol and ICRF‐159 were employed, significant deviations in the four survival curves became apparent. Continuous exposure to diethylstilboestrol and medroxyprogesterone acetate caused negligible cell kill. Due to these variable responses it was not possible to predict that any agent tested might have specific value in the treatment of prostate cancer. However, preliminary in vitro screening with such a panel of cell lines may complement in vivo studies with models of prostatic cancer and prove useful in investigations of mechanisms of drug resistance.

📜 SIMILAR VOLUMES

Differential patterns of anti-tumour dru

Differential patterns of anti-tumour drug responses and mechanisms of resistance in a series of independently-derived VP-16-resistant human tumour cell lines

✍ Richard B. Lock; Bridget T. Hill 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 French ⚖ 1010 KB

Resistance to etoposide, which was expressed following exposure of a human tumour cell line (HN-I) to fractionated X-irradiation (II fractions to a total dose of 50Gy), was found to be exhibited after delivery of only 5 fractions (total dose of 22.5Gy). In addition, 2 new etoposide-resistant subline

Involvement of Shc in the signaling resp

Involvement of Shc in the signaling response of human prostate tumor cell lines to epidermal growth factor

✍ John Gresham; Patricia Margiotta; Ann J. Palad; Kenneth D. Somers; Peter F. Blac 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 French ⚖ 134 KB 👁 2 views

Autocrine growth factors for the epidermal growth factor receptor (EGFR) have been identified in prostate tumors, implicating a role for EGFR in the progression of prostate cancer. To investigate early signaling mechanisms used by the EGFR in prostate tumor cells, we have characterized the involveme

In vitro response of human small-cell lu

In vitro response of human small-cell lung-cancer cell lines to chemotherapeutic drugs; no correlation with clinical data

✍ E. F. Smit; E. G. E. de Vries; H. Timmer-Bosscha; L. F. H. M. de Leij; J. W. Oos 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 French ⚖ 844 KB

Three cell lines derived from small-cell lung carcinoma (SCLC) tumors of patients who had no clinical response after treatment with a multi-drug regimen were compared to 3 cell lines derived from tumors of patients who, upon treatment, showed a complete clinical response. These 2 groups of cell line

Human prostatic carcinoma cell lines dis

Human prostatic carcinoma cell lines display altered regulation of polyamine transport in response to polyamine analogs and inhibitors

✍ Mi, Z.; Kramer, D.L.; Miller, J.T.; Bergeron, R.J.; Bernacki, R.; Porter, C.W. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 203 KB 👁 2 views

BACKGROUND. The possibility was investigated that complex homeostatic mechanisms which maintain polyamine pools in prostate-derived tumors may differ from those which are typically seen in other tissues and tumors. METHODS. Growth sensitivity and various regulatory responses were investigated in thr

Immortalization of a human colorectal ad

Immortalization of a human colorectal adenoma cell line by continuous in vitro passage: Possible involvement of chromosome 1 in tumour progression

✍ Christos Paraskrva; Susan Finerty; Susan Powell 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 French ⚖ 660 KB

A non-tumorigenic epithelial cell line designated PCIAA, derived from a large pre-malignant colorectal adenoma from a patient with familial polyposis coli (also referred to as hereditary adenomatosis of the colon and rectum) has become immortal in vitro. PCIAA has been passaged in vitro continuously

Endothelial CD44H mediates adhesion of a

Endothelial CD44H mediates adhesion of a melanoma cell line to quiescent human endothelial cells in vitro

✍ Elizabeth A. Price; Deirdre R. Coombe; J. Clifford Murray 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 French ⚖ 752 KB

A critical step in the metastatic spread of tumour cells is the interaction of circulating tumour cells with the vascular endothelium. We have investigated the role of CD44 and its variants in the adhesion of a human melanoma cell line (RPMI-795 I) and a breast adenocarcinoma cell line (MDA-MB-23 I)