Resonance assignment of 13C/15N labeled solid proteins by two- and three-dimensional magic-angle-spinning NMR
β Scribed by Mei Hong
- Book ID
- 110262312
- Publisher
- Springer Netherlands
- Year
- 1999
- Tongue
- English
- Weight
- 194 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0925-2738
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## Abstract ^13^C homonuclear throughβbond correlations of Ξ±β and Ξ²βchitin were determined by using twoβdimensional (2D) INADEQUATE spectra of these allomorphs purified from crab shell and squid pen, respectively. The 2D ^13^Cβ^13^C correlation spectra where two directly bonded carbons share a comm
We describe an approach to efficiently determine the backbone conformation of solid proteins that utilizes selective and extensive (13)C labeling in conjunction with two-dimensional magic-angle-spinning NMR. The selective (13)C labeling approach aims to reduce line broadening and other multispin com