The utility of gradient selection in MAS spectroscopy of dipolar solids is explored in two examples. In the first, rotor-synchronized gradients of appropriate strength and duration are applied to select 1H double-quantum coherences. The resulting DQ MAS spectrum of adamantane is compared with that a
โฆ LIBER โฆ
Direct Assignment of13C NMR Spectra of Rigid Solids by Two-Dimensional Magic-Angle-Spinning Separated-Local-Field Spectroscopy
โ Scribed by Hongjun Pan
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 123 KB
- Volume
- 124
- Category
- Article
- ISSN
- 1090-7807
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The assignment of nonexchanging protons of a small microcrystalline protein, the alpha-spectrin SH3 domain (7.2 kDa, 62 residues), was achieved by means of three-dimensional (3D) heteronuclear (1H-13C-13C) magic-angle spinning (MAS) NMR dipolar correlation spectroscopy. With the favorable combinatio