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Replicative and integrative plasmids for production of human interferon gamma in Bacillus subtilis

✍ Scribed by Juan A. Rojas Contreras; Mario Pedraza-Reyes; Leandro G. Ordoñez; Norma Urtiz Estrada; Ana P. Barba de la Rosa; Antonio De León-Rodríguez

Book ID
113855461
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
730 KB
Volume
64
Category
Article
ISSN
0147-619X

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Initiation of plasmid pC194 replication
Initiation of plasmid pC194 replication and its control in Bacillus subtilis
✍ Alonso, Juan C. ;Tailor, Ravindra H. 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 885 KB

By deletion analysis we have defined a 1.1 kb segment required for driving autonomous replication of the plasmid pC194. The minimal replicon specifies a positive, RepH, and a negative, Inc8A, trans-acting product and their target sites. The RepH product has a Mr of 34.1 kDa, could be overproduced, a