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Construction of a modular plasmid family for chromosomal integration in Bacillus subtilis

✍ Scribed by Gimpel, Matthias; Brantl, Sabine


Book ID
119326874
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
537 KB
Volume
91
Category
Article
ISSN
0167-7012

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The difficulty experienced in the shotgun cloning of chromosomal DNA on plasmid vectors in Bacillus subtilis is analyzed and an explanation for this difficulty is offered based on an inherent property of competent cells which imposes a requirement of plasmid multimers in transformation of plasmid-fr