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Replication of the tetracycline resistance plasmid pSC101

✍ Scribed by G. Churchward; Xia Guixan; T. Goebel; D. Manen; E. Othon-Izaurraulde; L. Caro

Publisher: Springer
Year: 1986
Tongue: English
Weight: 142 KB
Volume: 42
Category: Article
ISSN: 1420-682X
DOI: 10.1007/bf01975992

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Altered tetracycline resistance in pSC10

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Investigation of tetracycline resistance genetically determined by the plasmid pSC101 and several recombinants of pSC101 containing EcoRI generated DNA fragments inserted at the EcoRI site has revealed significant differences in the phenotypic expression of that resistance. The altered phenotypes of

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Two regions tentatively called unsA and unsR were identified on pSC101. One, unsA, corresponds to less than 650 bp of the N-terminal in the tetracycline resistance structural gene and seems to inhibit stable maintenance of pSC101. The other, unsR, is defined within the 1 kb XhoI-EcoRI region located

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The rate of replication of the plasmids colE1, pSC101, R100.1 and pAR132 (an RTF-TC derivative of the drug resistance factor R100.1) has been investigated directly by DNA:DNA hybridization. These rates have been compared, in a dnaAts strain, to that of various markers of the host chromosome at permi