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Altered tetracycline resistance in pSC101 recombinant plasmids

โœ Scribed by Tait, Robert C. ;Rodriguez, Raymond L. ;Boyer, Herbert W.

Publisher
Springer
Year
1977
Tongue
English
Weight
457 KB
Volume
151
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

Investigation of tetracycline resistance genetically determined by the plasmid pSC101 and several recombinants of pSC101 containing EcoRI generated DNA fragments inserted at the EcoRI site has revealed significant differences in the phenotypic expression of that resistance. The altered phenotypes of the recombinant plasmids may be the result of the location of the EcoRI site of pSC101, which has been determined to be near the genetic elements involved with tetracycline resistance.

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Regions associated with the stable maint
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โœ Makino, Souichi ;Sasakawa, Chihiro ;Dambara, Hirofumi ;Yoshikawa, Masanosuke ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› Springer ๐ŸŒ English โš– 517 KB

Two regions tentatively called unsA and unsR were identified on pSC101. One, unsA, corresponds to less than 650 bp of the N-terminal in the tetracycline resistance structural gene and seems to inhibit stable maintenance of pSC101. The other, unsR, is defined within the 1 kb XhoI-EcoRI region located

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