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Regions associated with the stable maintenance of plasmid pSC101 and its tetracycline resistance

✍ Scribed by Makino, Souichi ;Sasakawa, Chihiro ;Dambara, Hirofumi ;Yoshikawa, Masanosuke

Publisher: Springer
Year: 1986
Tongue: English
Weight: 517 KB
Volume: 204
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00330197

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✦ Synopsis

Two regions tentatively called unsA and unsR were identified on pSC101. One, unsA, corresponds to less than 650 bp of the N-terminal in the tetracycline resistance structural gene and seems to inhibit stable maintenance of pSC101. The other, unsR, is defined within the 1 kb XhoI-EcoRI region located upstream of the tetracycline resistance structural gene and is a regulatory gene clearly distinct from tetR (Unger et al. 1984); it serves as a suppressor of the unsA function.

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