Replication of mini-F plasmids during the bacterial division cycle
β Scribed by Keasling, J.D; Palsson, B.O; Cooper, S
- Book ID
- 123154740
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 523 KB
- Volume
- 143
- Category
- Article
- ISSN
- 0923-2508
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We constructed a series of defective mini-F plasmids, which have deletion(s) in the replication origin I and/or origin II, and their derivatives, which do not produce F3 protein, by insertion of the XhoI fragment of Tn5 into the XhoI site at 41.0 F (kilobases on the coordinate map of F-plasmid). Usi
We isolated a series of Tn5-insertional mutants from the mini-F plasmid, which has a deletion in the origin II region and replicates exclusively from origin 1, and found that the mutants that had Tn5 in either the F4 or the F5 gene were defective in their replication. It is concluded that, in additi
At the XhoI site (45.08F) of plasmid mini-F a deletion of 649 bp was generated employing exonuclease Bal31. By this deletion nucleotide sequences functioning as origin II and the four 19 bp direct repeats constituting the incB region in front of the E protein gene were removed from the plasmid. Anal
Provides An Unique Overview On Bacterial Genetics, Bacterial Genome Projects And Gene Technology And Its Applicaitons In Biological And Biomedical Research And Medicine. The Author Guides The Reader Up The Front In Research Within The Different Fields Of Bacterial Genetics, Based Mainly On Results R