A simple, rapid, and inexpensive procedure for the determination of aminoacyl-tRNA synthetase activity is described. The assay is based on a green color formed between PPi and ammonium molybdate in the presence of mercaptoethanol. The sensitivity is in the nanomole range and is comparable with the c
Repeating sequences in aminoacyl-tRNA synthetases
โ Scribed by Koch, G.L.E.; Boulanger, Y.; Hartley, B.S.
- Book ID
- 109690470
- Publisher
- Nature Publishing Group
- Year
- 1974
- Tongue
- English
- Weight
- 710 KB
- Volume
- 249
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/249316a0
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๐ SIMILAR VOLUMES
The aminoacyl-tRNA synthetases are an ancient group of enzymes that catalyze the covalent attachment of an amino acid to its cognate transfer RNA. The question of specificity, that is, how each synthetase selects the correct individual or isoacceptor set of tRNAs for each amino acid, has been referr
A simple, continuous assay for aminoacyl-tRNA synthetases utilizing a commercially available pyrophosphate assay reagent kit was demonstrated. The method coupled aminoacyl-tRNA synthetase activity with pyrophosphate-dependent fructose-6-phosphate kinase, aldolase, triosephosphate isomerase, and glyc