Repeated hybridoma batch culture with cell recycle
โ Scribed by H. D. Blasey; A. R. Bernard
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 229 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0920-9069
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The acetic acid concentration in a batch culture of Acetobacter aceti M23 increased up to 90 g/l by adding ethanol intermittently. Although the bacterial cells ceased growth at about 60 g acetic acid/l, non-viable cells still preserved ethanol oxidation activity. Cell recycling by filtration in a re
We have developed a high cell density and high product concentration culture system recycling high molecular weight components. The production of monoclonal antibodies in high concentration was performed by this culture system with mouse human hybridoma H2 and V6 cells in serum-free defined media.