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High cell density perfusion culture of hybridoma cells recycling high molecular weight components

โœ Scribed by Yoshiharu Takazawa; Michiyuki Tokashiki; Kimihiko Hamamoto; Hiroki Murakami

Publisher
Springer
Year
1988
Tongue
English
Weight
480 KB
Volume
1
Category
Article
ISSN
0920-9069

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โœฆ Synopsis

We have developed a high cell density and high product concentration culture system recycling high molecular weight components. The production of monoclonal antibodies in high concentration was performed by this culture system with mouse human hybridoma H2 and V6 cells in serum-free defined media.

The concentration of IgG after 48 days culture of H2 cells in ITES-eRDF reached 2 mg/ml and the purity of IgG in culture fluid was 61%. In addition, high molecular weight components in serum-free media, such as transferrin or BSA, could be reduced to 5% of the original concentration.

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High cell density perfusion culture of mouse-human hybridomas
โœ Yoshiharu Takazawa; Michiyuki Tokashiki ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› Springer ๐ŸŒ English โš– 409 KB

Five mouse-human hybridomas, H2, H3, V1, V2 and V6 cells secreting anti-virus human monoclonal immunoglobulin G (IgG) were cultured in serum-free media at high density in a settling perfusion culture vessel with an inner cell sedimentation zone. The H2, H3 and V6 cells reached a density of 107 cells

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โœ Michiyuki Tokashiki; Takami Arai; Kimihiko Hamamoto; Kenji Ishimaru ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 368 KB

The influence of centrifugal force on the growth of cells was examined by exposing the cells of the mouse-human hybridoma X87 line to centrifugal force (100-500 G) for ten minutes twice a day and comparing the static culture with that of unexposed cells. In this experiment, both cell proliferation a

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โœ Michiyuki Tokashiki; Takami Arai ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› Springer ๐ŸŒ English โš– 248 KB

Mouse-human hybridoma X87X ceils were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0 x 10 v cells/ml, when the