High density culture of hybridoma cells in a dual hollow fiber bioreactor
β Scribed by Duk Jae Oh; Ho Nam Chang
- Publisher
- Springer-Verlag
- Year
- 1992
- Tongue
- English
- Weight
- 259 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0951-208X
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## Abstract Demand for increasingly complex postβtranslationally modified proteins, such as monoclonal antibodies (mAbs), necessitates the use of mammalian hosts for production. The focus of this article is a continuous centrifugal bioreactor (CCBR) capable of increasing volumetric productivity for
VERO cells were cultivated in microcarriers (MCs) using a bioreactor with a working capacity of 3.7 1. We studied the effect of MCs concentration in the proportion of MC-bearing cells and on the kinetics of cell growth, as well as the cell growth with a batch, fed-batch and perfusion mode operation.
The influence of centrifugal force on the growth of cells was examined by exposing the cells of the mouse-human hybridoma X87 line to centrifugal force (100-500 G) for ten minutes twice a day and comparing the static culture with that of unexposed cells. In this experiment, both cell proliferation a