Mechanical properties of hybridoma cells in batch culture
โ Scribed by Z. Zhang; M. Al-Rubeai; C. R. Thomas
- Publisher
- Springer Netherlands
- Year
- 1992
- Tongue
- English
- Weight
- 325 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0141-5492
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We have studied the effect of the pyruvate dehydrogenase (PDH) activator, dichloroacetate (DCA), on the growth, metabolism, and productivity of the PQXB 1/2 hybridoma cell line. In control batch cultures, cessation of growth and the onset of decline phase coincided with the time at which the media b
Cell culture longevity in fed-batch culture of hybridomas is often limited by elevated medium osmolality caused by repeated nutrient feeding. Shotwise feeding of 10ร Dulbecco's modified Eagle's medium (DMEM) concentrates elevated the osmolality of medium up to 540 mOsm/kg at the end of fed-batch cul