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Removal of sodium dodecyl sulfate from proteins

โœ Scribed by Oscar H. Kapp; Serge N. Vinogradov

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
383 KB
Volume
91
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Effective removal of sodium dodecyl sulfate from proteins in water or sodium phosphate buffer was achieved by column chromatography using the ion-retardation resin AG11A8. An average recovery of 83% protein was obtained, while 0.1 to 1.4 moles of sodium dodecyl sulfate remained on each mole of protein.

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