Effective removal of sodium dodecyl sulfate from proteins in water or sodium phosphate buffer was achieved by column chromatography using the ion-retardation resin AG11A8. An average recovery of 83% protein was obtained, while 0.1 to 1.4 moles of sodium dodecyl sulfate remained on each mole of prote
Removal of dodecyl sulfate from protein solution
โ Scribed by Haruo Suzuki; Tomohiko Terada
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 375 KB
- Volume
- 172
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
Sodium dodecyl sulfate is one of the most widely used detergents. But, there is no good method to remove dodecyl sulfate (DS) from a small volume of protein solution. We showed that (a) DS in protein solution was removed by the addition of potassium salts with high recovery of protein, and (b) the method was useful before tryptic digestion of protein. o 1988 Academic Press, Inc.
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A simple method and apparatus are described for the efficient recovery of proteins from sodium dodecyl sulfate-polyacrylamide gel systems after electrophoretic resolution. This procedure provides for high yields of proteins which are free of sodium dodecyl sulfate and in certain cases, exhibit signi