๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Removal of endotoxin from protein solutions by phase separation using triton X-114

โœ Scribed by Yoshitomi Aida; Michael J. Pabst

Book ID
118011418
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
359 KB
Volume
132
Category
Article
ISSN
0022-1759

No coin nor oath required. For personal study only.

๐Ÿ“œ SIMILAR VOLUMES

Removal of endotoxin from protein soluti
Removal of endotoxin from protein solutions by phase separation using triton X-114
โœ Yoshitomi Aida; Michael J. Pabst ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 359 KB

Endotoxin contamination of protein solutions was reduced by a phase separation technique using the detergent, Triton X-114. Protein solutions containing endotoxin were treated with Triton X-114 on ice. The solution was then warmed to 37ยฐC, whereupon two phases formed. The Triton X-114 phase, contain

Phase separation of rat intestinal brush
Phase separation of rat intestinal brush border membrane proteins using Triton X-114
โœ Chinnaswamy Tiruppathi; David H. Alpers; Bellur Seetharam ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 901 KB

Rat intestinal microvillus membrane contains at least 24 polypeptides, of which 18 can be solubilized using Triton X-114 at 4 degrees C. Upon phase separation at 32 degrees C, 11 proteins separated nearly completely into the detergent-rich phase, while 9 proteins were found exclusively in the aqueou

Phase separation temperatures of mixture
Phase separation temperatures of mixtures of Triton X-114 and Trition X-45: Application to protein separation
โœ Barry R. Ganong; John P. Delmore ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 333 KB

Triton X-114 solutions separate above 22 degrees C into two immiscible aqueous phases. The more dense phase is enriched in detergent, and the less dense phase is depleted of detergent, relative to the original single phase. This phenomenon has been used to partition proteins according to hydrophobic