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Isolation of lumenal proteins from spinach thylakoid membranes by Triton X-114 phase partitioning

✍ Scribed by Terry M Bricker; Mandy Prevost; Vivian Vu; Susan Laborde; Johnna Womack; Laurie K Frankel

Book ID
117046295
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
552 KB
Volume
1503
Category
Article
ISSN
0005-2728

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Removal of endotoxin from protein soluti
Removal of endotoxin from protein solutions by phase separation using triton X-114
✍ Yoshitomi Aida; Michael J. Pabst πŸ“‚ Article πŸ“… 1990 πŸ› Elsevier Science 🌐 English βš– 359 KB

Endotoxin contamination of protein solutions was reduced by a phase separation technique using the detergent, Triton X-114. Protein solutions containing endotoxin were treated with Triton X-114 on ice. The solution was then warmed to 37Β°C, whereupon two phases formed. The Triton X-114 phase, contain