Isolation of Photosystem II enriched membrane vesicles from spinach chloroplasts by phase partition
✍ Scribed by Hans-Erik Åkerlund; Bertil Andersson; Per-Åke Albertsson
- Book ID
- 115716778
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 811 KB
- Volume
- 449
- Category
- Article
- ISSN
- 0005-2728
No coin nor oath required. For personal study only.
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Ion exchange perfusion chromatography has been teins requires the presence of detergent to solubilize introduced for the isolation of hydrophobic membrane the proteins from the lipid bilayer and to keep them protein complexes from thylakoid membranes of spinin solution during subsequent isolation. H
An improved, non-detergent, method for preparative isolation of PS II membrane vesicles from spinach chloroplasts is presented. Thylakoids (chlorophyll (Chl) a/b ratio 2.8, Chl/P700 435) were fractionated by Yeda press treatment and aqueous two-phase partition to yield inside-out vesicles (1) (chl a