𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Preparation of highly enriched photosystem II membrane vesicles by a non-detergent method

✍ Scribed by Per Svensson; Per-Åke Albertsson

Book ID
104615797
Publisher
Springer
Year
1989
Tongue
English
Weight
603 KB
Volume
20
Category
Article
ISSN
0166-8595

No coin nor oath required. For personal study only.

✦ Synopsis

An improved, non-detergent, method for preparative isolation of PS II membrane vesicles from spinach chloroplasts is presented. Thylakoids (chlorophyll (Chl) a/b ratio 2.8, Chl/P700 435) were fractionated by Yeda press treatment and aqueous two-phase partition to yield inside-out vesicles (1) (chl a/b 2.2, chl/P700 700). These vesicles were subjected a sonication--phase partitioning procedure; steps of sonication of inside-out vesicles, while still present in a dextran-polyethylene glycol two-phase system were alternated by phase partition. These steps selectively removed P700-containing membrane fragments from the inside-out vesicles and yielded a membrane fraction with improved PSII purity (Chl a/b ratio 1.9, Chl/P700 1500) and retained oxygen evolving capacity (295 #mo102 mg Chl-~ h-1).

📜 SIMILAR VOLUMES

Characterization of a photosystem II rea
Characterization of a photosystem II reaction center complex isolated by exposure of PSII membranes to a non-ionic detergent and high concentrations of NaCl
✍ Demetrios F. Ghanotakis; Charles F. Yocum 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 265 KB

A highly resolved PSII reaction center complex has been prepared by exposure of PSII membranes to the detergent octylglucopyranoside at elevated ionic strengths; oxygen evolution activity is about i,OOO ~moles Og/hr/mg Ch] in the presence of CaCl 9. A Mn quantitation and ~ kinetic study of Z, the d

A Novel Method of Preparation of Small I
A Novel Method of Preparation of Small Intestinal Brush Border Membrane Vesicles by Polyethylene Glycol Precipitation
✍ R. Prabhu; K.A. Balasubramanian 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 65 KB

A novel method of brush border membrane vesicle (BBMV) preparation from the small intestinal mucosa using polyethylene glycol (PEG) precipitation has been presented. This preparation is compared with calcium-precipitated BBMVs in marker enzyme enrichment, contamination by other subcellular membranes