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Characterization of a photosystem II reaction center complex isolated by exposure of PSII membranes to a non-ionic detergent and high concentrations of NaCl

✍ Scribed by Demetrios F. Ghanotakis; Charles F. Yocum


Book ID
104627940
Publisher
Springer
Year
1986
Tongue
English
Weight
265 KB
Volume
10
Category
Article
ISSN
0166-8595

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✦ Synopsis


A highly resolved PSII reaction center complex has been prepared by exposure of PSII membranes to the detergent octylglucopyranoside at elevated ionic strengths; oxygen evolution activity is about i,OOO ~moles Og/hr/mg Ch] in the presence of CaCl 9.

A Mn quantitation and ~ kinetic study of Z, the donor to P'8-' reveals that on a Chl basis this new preparation showsba~ almost four-fold enrichment in Mn and the electron transport components of PSII.

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