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Removing endotoxin from plasmid samples by Triton X-114 isothermal extraction

โœ Scribed by Rui Ma; Jun Zhao; Hui-Cong Du; Shuang Tian; Li-Wen Li


Book ID
113412426
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
286 KB
Volume
424
Category
Article
ISSN
0003-2697

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๐Ÿ“œ SIMILAR VOLUMES


Removal of endotoxin from protein soluti
โœ Yoshitomi Aida; Michael J. Pabst ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 359 KB

Endotoxin contamination of protein solutions was reduced by a phase separation technique using the detergent, Triton X-114. Protein solutions containing endotoxin were treated with Triton X-114 on ice. The solution was then warmed to 37ยฐC, whereupon two phases formed. The Triton X-114 phase, contain