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Reliable detection of St. Louis encephalitis virus by RT-nested PCR

✍ Scribed by Viviana Ré; Lorena Spinsanti; Adrián Farías; Adrián Díaz; Ana Vázquez; Javier Aguilar; Antonio Tenorio; Marta Contigiani

Book ID
119890861
Publisher
Elsevier Science
Year
2008
Tongue
Spanish
Weight
146 KB
Volume
26
Category
Article
ISSN
0213-005X

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## Abstract Using the RT‐PCR with primers that anneal to the 5′ and the 3′ extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of thr