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Development and field evaluation of a nested RT-PCR kit for detecting Japanese encephalitis virus in mosquitoes

✍ Scribed by Young Eui Jeong; Min Ju Jeon; Jung Eun Cho; Myung Guk Han; Hwan Ju Choi; Mi Yeong Shin; Hag Jae Park; Woosik Kim; Bong Chun Moon; Ji-Sung Park; Bona Park; Young Ran Ju


Book ID
113768553
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
407 KB
Volume
171
Category
Article
ISSN
0166-0934

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## Abstract A dengue‐3‐specific real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR) was developed using the novel __L__ight __U__pon e__X__tension (LUXβ„’) fluorogenic technology. A labeled forward primer and a standard reverse primer that target a conserved region within the non‐struc