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Development and evaluation of a real-time RT-PCR assay for Sindbis virus detection

✍ Scribed by Jussi Sane; Satu Kurkela; Lev Levanov; Simo Nikkari; Antti Vaheri; Olli Vapalahti

Book ID: 113768820
Publisher: Elsevier Science
Year: 2012
Tongue: English
Weight: 468 KB
Volume: 179
Category: Article
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2011.10.021

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## Abstract A dengue‐3‐specific real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR) was developed using the novel __L__ight __U__pon e__X__tension (LUX™) fluorogenic technology. A labeled forward primer and a standard reverse primer that target a conserved region within the non‐struc