Diagnosis of Oropouche virus infection by RT-nested-PCR

✍ Scribed by Marcos Lázaro Moreli; Victor Hugo Aquino; Ana Cecília R. Cruz; Luiz Tadeu M. Figueiredo

• Book ID: 102378134
• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 124 KB
• Volume: 66
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.2122

✦ Synopsis

Abstract

Using the RT‐PCR with primers that anneal to the 5′ and the 3′ extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show that this RT‐nested‐PCR is a useful tool for rapid diagnosis of Oropouche fever infections. J. Med. Virol. 66:139–142, 2002. © 2002 Wiley‐Liss, Inc.