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Diagnosis of canine coronavirus infection using nested-PCR

✍ Scribed by Annamaria Pratelli; Domenico Buonavoglia; Vito Martella; Maria Tempesta; Antonio Lavazza; Canio Buonavoglia

Book ID
114140859
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
62 KB
Volume
84
Category
Article
ISSN
0166-0934

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## Abstract Using the RT‐PCR with primers that anneal to the 5β€² and the 3β€² extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of thr