Relation between sister chromatid exchange, cell proliferation and proportion of B and T cells in human lymphocyte cultures
โ Scribed by Annette Lindblad; Bo Lambert
- Publisher
- Springer
- Year
- 1981
- Tongue
- English
- Weight
- 368 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0340-6717
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๐ SIMILAR VOLUMES
Lymphocytes from 20 notmal subjects (11 male and 9 female) were examined for the frequency and location of sister chromatid exchanges (SCE) by the BrdU--Giemsa method. The mean frequency of SCE was 6.37 with little significant variation. One subject had a high number of exchanges in chromosome 1 whi
Human leukocyte cultures were pulse-treated with the trifunctional alkylating mutagen trenimon in a final concentration of 10(-7) M for 15--20 h after culture start, i.e., in the G1 phase of the cell cycle. At 24 h after culture start bromodeoxyuridine (BUdR) was added to the trenimon-treated cultur