Hydroxybutyryl-Coenzym A (4-Hydroxybutyryl-CoA, 1, Schema 1) enthält drei diastereotope Methylengruppen, die während der reversiblen Dehydratisierung zu Crotonyl-CoA (But-2-enoyl-CoA, 2) entweder ein Wasserstoffatom verlieren oder hinzugewinnen. Die 4-Hydroxybutyryl-CoA-Dehydratase aus Clostridium a
Reinigung von Coenzym A und Isolierung von reinem Dephospho-Coenzym A
✍ Scribed by Olga Brenner-Holzach; R. Adler; F. Leuthardt
- Publisher
- John Wiley and Sons
- Year
- 1956
- Tongue
- German
- Weight
- 445 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0018-019X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
CoA (Pabst Laboratories, Milwaukee) was purified by chromatography on Dowex 1 columns. To obtain a good separation from the byproducts it is essential to use a resin of a low cross‐linking (4%).
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