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Regulation of glucose transport in chick fibroblasts: Bicarbonate, lactate and ascorbic acid

✍ Scribed by H. Amos; C. W. Christopher; T. A. Musliner

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
515 KB
Volume
89
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

The rate at which chick embryo fibroblasts in primary or secondary culture transport glucose or 3‐O‐methyl glucose is strongly influenced by the presence of bicarbonate ion in the culture medium. Cells growing or maintained on glucose at physiologic concentration (5.5mM) have an 8 to 10‐fold higher rate of glucose uptake than their counterparts cultivated without bicarbonate. These cells also produce more lactate as a consequence of their more rapid intake of glucose. The hydrogen acceptors, methylene blue and dehydroascorbate added to the culture medium reduce the cell capacity to transport glucose and 3‐O‐methyl glucose to levels obtaining in the bicarbonate‐free medium. There is a concomitant reduction in glucose utilized by cells during 24 hours and a further reduction in lactate formed per molecule of glucose metabolized.

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