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Regulation of glucose utilization in chick embryo fibroblasts by bicarbonate ion

✍ Scribed by Mary H. Miller; Harold Amos; Donald A. Sens

Publisher: John Wiley and Sons
Year: 1981
Tongue: English
Weight: 620 KB
Volume: 107
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041070216

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✦ Synopsis

Abstract

The amount of glucose consumed by chick embryo fibroblasts in primary culture is strongly influenced by the presence of bicarbonate ion in the culture medmum. Cells grown on glucose at physiologic concentration (5.5 mm) and in the absence of bicarbonate ion have a reduced rate of glucose utilization when compared to their counterparts cultivated in medium containing the usual 25 mM bicarbonate. The presence or absence of bicarbonate is without effect on chick embryo fibroblast proliferation over a 6‐day growth period. Both lactic acid accumulation per mole of glucose consumed and the utilization of glutamine increase as a function of bicarbonate ion in the growth medium.

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