Regulation of Bacteriophage λ Development by Guanosine 5′-Diphosphate-3′-diphosphate
✍ Scribed by Monika Słomińska; Peter Neubauer; Grzegorz Węgrzyn
- Book ID
- 112272762
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 223 KB
- Volume
- 262
- Category
- Article
- ISSN
- 0042-6822
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A method is described for the rapid analysis of the nucleotides, guanosine S-diphosphate-3'-diphosphate (ppGpp) and guanosine 5'-triphosphate-3'-diphosphate (pppGpp), by high-performance liquid chromatography. It has been found that the inclusion of magnesium acetate in the potassium phosphate buffe
The in vitro synthesis of ribonucleic acid (RNA) by S-30 extracts of Escherichia coli K-12 is stimulated from two-to fourfold by 0.16 mM to 0.32 mM guanosine 5'-diphosphate 3'-diphosphate (ppGpp) when either gammacI857St68h80 deoxyribonucleic acid (gammah80 DNA), gammah80dilv DNA or gammah80dlac DNA