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A rapid method for the determination of guanosine 5′-diphosphate-3′-diphosphate and guanosine 5′-triphosphate-3′-diphosphate by high-performance liquid chromatography

✍ Scribed by Meir Fischer; Thomas P. Zimmerman; Steven A. Short

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
380 KB
Volume
121
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method is described for the rapid analysis of the nucleotides, guanosine S-diphosphate-3'-diphosphate (ppGpp) and guanosine 5'-triphosphate-3'-diphosphate (pppGpp), by high-performance liquid chromatography. It has been found that the inclusion of magnesium acetate in the potassium phosphate buffer facilitates elution of these highly phosphorylated compounds from the Partisil strong anion-exchange resin and allows their determination under isocratic conditions. The application of this methodology to formic acid extracts of bacterial cells is demonstrated.

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