✦ LIBER ✦

Regeneration of transgenic tamarillo plants

✍ Scribed by Ross G. Atkinson; Richard C. Gardner

Publisher: Springer
Year: 1993
Tongue: English
Weight: 795 KB
Volume: 12
Category: Article
ISSN: 0721-7714
DOI: 10.1007/bf00237433

No coin nor oath required. For personal study only.

✦ Synopsis

Media were developed to regenerate shoots from leaf pieces of tamarillo (Cyphomandra betacea (Cav.) Sendtner). Shoots were derived via organogenesis and could be easily rooted and transferred to the growth chamber. Transgenic tamarillo plants were produced using the binary vector pKIWI110 in the avirulent Agrobacterium strain LBA4404. All transgenic plants were kanamycin resistant and some plants expressed the β D-glucuronidase (gusA) reporter gene and were chlorsulfuron resistant. Molecular evidence for transformation was obtained using PCR (polymerase chain reaction) and Southern hybridization. Inheritance of the transgenic phenotypes was demonstrated in seedling progeny.

📜 SIMILAR VOLUMES

Transformation of pecan and regeneration

Transformation of pecan and regeneration of transgenic plants

✍ Gale H. McGranahan; Charles A. Leslie; Abhaya M. Dandekar; Sandra L. Uratsu; Ida 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 900 KB

Agrobacterium-mediated transformation of

Agrobacterium-mediated transformation of pepino and regeneration of transgenic plants

✍ Roos G. Atkinson; Richard C. Gardner 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 880 KB

Regeneration of pepino (Solanum muricatum Ait.) shoots was achieved both by organogenesis and by embryogenesis. Shoots derived via organogenesis were easily rooted and most regenerated plants appeared phenotypically normal. Transgenic plants were obtained using the binary vector pKIWI110 in the avir

Agrobacterium-mediated transformation of

Agrobacterium-mediated transformation ofCitrusstem segments and regeneration of transgenic plants

✍ G. A. Moore; C. C. Jacono; J. L. Neidigh; S. D. Lawrence; K. Cline 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 725 KB

Electroporation of immature maize zygoti

Electroporation of immature maize zygotic embryos and regeneration of transgenic plants

✍ Ke Xiayi; Zhang Xiuwen; Shi Heping; Li Baojian 📂 Article 📅 1996 🏛 Springer Netherlands 🌐 English ⚖ 628 KB

Transformation of grape (Vitis viniferaL

Transformation of grape (Vitis viniferaL.) zygotic-derived somatic embryos and regeneration of transgenic plants

✍ R. Scorza; J. M. Cordts; D. W. Ramming; R. L. Emershad 📂 Article 📅 1995 🏛 Springer 🌐 English ⚖ 989 KB

Transgenic grape plants were regenerated from somatic embryos derived from immature zygotic embryos of seedless grape (Vitis vinifera L.) selections. Somatic embryos were bombarded twice with 1 μm gold particles using the Biolistic PDS-1000/He device (Bio-Rad Laboratories) and then exposed to Agroba

Stable transformation via electroporatio

Stable transformation via electroporation into maize Type II callus and regeneration of fertile transgenic plants

✍ S. M. Pescitelli; K. Sukhapinda 📂 Article 📅 1995 🏛 Springer 🌐 English ⚖ 936 KB

Maize Type II callus tissue was used as the plant material for genetic transformation via electroporation. Plasmid DNA containing a selectable marker gene (either neomycin phosphotransferase (npt-II) or phosphinothricin acetyl transferase (bar)), and a screenable marker gene (gus A) was incubated wi