Transformation of grape (Vitis viniferaL.) zygotic-derived somatic embryos and regeneration of transgenic plants

✍ Scribed by R. Scorza; J. M. Cordts; D. W. Ramming; R. L. Emershad

• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 989 KB
• Volume: 14
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00231944

✦ Synopsis

Transgenic grape plants were regenerated from somatic embryos derived from immature zygotic embryos of seedless grape (Vitis vinifera L.) selections. Somatic embryos were bombarded twice with 1 μm gold particles using the Biolistic PDS-1000/He device (Bio-Rad Laboratories) and then exposed to Agrobacterium tumefaciens strain C58/Z707 containing the binary plasmid pGA482GG or pCGN7314. Following cocultivation, secondary embryos were allowed to proliferate on Emershad/Ramming proliferation (ERP) medium for 6 weeks before selection on ERP medium containing 20-40 μg/ml kanamycin (kan). Transgenic embryos were identified after 3-5 months under selection and allowed to germinate and develop into rooted plants on Woody Plant Medium containing 1 μM 6-benzylaminopurine (BAP), 1.5% sucrose, 0.3% activated charcoal and 0.75% agar. Integration of the foreign genes into these grapevines was verified by growth in the presence of kan, positive GUS and PCR assays, and Southern analysis.