Plant regeneration from mesophyll protop
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Samir R. Sikdar; Soma Sengupta; Srabani Das; S. K. Sen
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Article
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1990
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Springer
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English
β 635 KB
Mesophyll protoplasts from leaves of aseptically grown shoot tips of Diplotaxis muralis were isolated (6.2-7.1Γ10(5) protoplasts/g fresh weight of tissue) using one step enzyme digestion. The protoplasts (71% viability) underwent divisions (4.2+0.1%) on plating in M8PS2 medium and ultimately formed