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Plant regeneration from protoplasts of a wild lettuce species (Lactuca salignaL.)

โœ Scribed by C. Brown; J. A. Lucas; J. B. Power


Publisher
Springer
Year
1987
Tongue
English
Weight
264 KB
Volume
6
Category
Article
ISSN
0721-7714

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๐Ÿ“œ SIMILAR VOLUMES


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Mesophyll protoplasts from leaves of aseptically grown shoot tips of Diplotaxis muralis were isolated (6.2-7.1ร—10(5) protoplasts/g fresh weight of tissue) using one step enzyme digestion. The protoplasts (71% viability) underwent divisions (4.2+0.1%) on plating in M8PS2 medium and ultimately formed

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A protocol has been established for rapid, high frequency plant regeneration from protoplasts of the wild tomato species Lycopersicon chilense Dun. Cell suspension cultures were obtained from calli initiated from seedling stem explants. Protoplasts were isolated from cell suspensions by an overnight