We have assessed the capacity of cultured protoplasts from two tissue sources of several commercially-grown broccoli cultivars to regenerate plants. A procedure that employs hypocotyl protoplasts and a culture medium with a high NAA:2,4-D auxin ratio was developed. The procedure permits highly effic
Regeneration of plants from hypocotyl protoplasts of rapessed (Brassica napusL. var.oleifera) cultivars
✍ Scribed by Gert B. Poulsen; Søren V. S. Nielsen
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 279 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0167-6857
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✦ Synopsis
Protoplast cultures were prepared from hypocotyls of ten spring rapeseed cultivars. Protoplasts from all genotypes tested formed caUi, and shoots were regenerated from calli of nine of the genotypes at frequencies varying from 15 to 76%. The regenerating cultivars fell into a high regenerating group (> 60%) and a low regenerating group < 25%).
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A sunflower genotype (Helianthus annuus L. cv. Florom-328) able to regenerate plants from in vitro cultures was identified by screening hybrids and inbred lines. Protoplasts of this genotype were isolated from dark grown hypocotyls and were cultured in droplets of agarose-solidified V-KM medium cove
Three Indian cultivars of L. esculentum were assessed for shoot regeneration from protoplast-derived calli. Consistent yields of viable protoplasts (> 9.0 x 106 g f.wt. -1) were obtained from leaflets of 14 days old cultured shoots. Protoplast viability (88-94%) and plating efficiency (55-70%) were