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Highly efficlent plant regeneration from mesophyll protoplasts of Indian field cultivars of tomato (Lycopersicon esculentum)

✍ Scribed by R. S. Patil; M. R. Davey; J. B. Power


Publisher
Springer
Year
1994
Tongue
English
Weight
249 KB
Volume
36
Category
Article
ISSN
0167-6857

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✦ Synopsis


Three Indian cultivars of L. esculentum were assessed for shoot regeneration from protoplast-derived calli. Consistent yields of viable protoplasts (> 9.0 x 106 g f.wt. -1) were obtained from leaflets of 14 days old cultured shoots. Protoplast viability (88-94%) and plating efficiency (55-70%) were recorded for the three cultivars. Up to 71% of the protoplast-derived tissues regenerated shoots.